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🔬 Laboratory · 6 min

Rapid diagnostic tests (RDTs): doing them right

A rapid diagnostic test (RDT) gives a result in minutes, often outside a laboratory. It is simple to do, but each step still counts. Small mistakes can change the result.

Before the test

  • Read the insert. Each test has its own steps, volumes and timing, so never work from memory or from another brand's habits.
  • Check the expiry date and the lot number. Do not use an expired test.
  • Check the pouch is sealed and undamaged, and the drying agent (desiccant) is present if the insert includes one. Open the pouch only when you are ready to start.
  • Store tests exactly as the insert says, away from sun, heat and damp.

Doing the test

  • Add the sample and the buffer in the amounts and order the insert gives. Use the device on a flat surface.
  • Start a timer. Read the result inside the time window in the insert, not earlier and not later. An early read can miss a weak positive. A late read can show lines that mean nothing.
  • Look at the control line first. If the control line does not appear, the test is invalid, whatever the other line shows. Do not report a result. Repeat with a new device and check the lot, the expiry date, the storage and your steps.
  • Never reuse a device. One device, one test, one patient.

After the test

  1. Record the patient ID, result, test name, lot, expiry date, date, time and your name.
  2. Dispose of the used device as infectious waste.
  3. If your national testing guidance requires a confirmatory step for certain results, follow it. Do not give a first result as final when confirmation is required.
Tip: A control line that does not show up is not bad luck. It is the test telling you not to trust that device.

Sources: WHO guidance on the use and quality assurance of rapid diagnostic tests (current edition) · Manufacturer's instructions for use for each RDT · National testing algorithms and guidelines of the Ministry of Health (current)

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For licensed professionals and students. Educational information only: always follow current national guidelines (EFDA, Ministry of Health), the product label and your facility’s procedures.