🔬 Laboratory · 6 min
Rapid diagnostic tests (RDTs): doing them right
A rapid diagnostic test (RDT) gives a result in minutes, often outside a laboratory. It is simple to do, but each step still counts. Small mistakes can change the result.
Before the test
- Read the insert. Each test has its own steps, volumes and timing, so never work from memory or from another brand's habits.
- Check the expiry date and the lot number. Do not use an expired test.
- Check the pouch is sealed and undamaged, and the drying agent (desiccant) is present if the insert includes one. Open the pouch only when you are ready to start.
- Store tests exactly as the insert says, away from sun, heat and damp.
Doing the test
- Add the sample and the buffer in the amounts and order the insert gives. Use the device on a flat surface.
- Start a timer. Read the result inside the time window in the insert, not earlier and not later. An early read can miss a weak positive. A late read can show lines that mean nothing.
- Look at the control line first. If the control line does not appear, the test is invalid, whatever the other line shows. Do not report a result. Repeat with a new device and check the lot, the expiry date, the storage and your steps.
- Never reuse a device. One device, one test, one patient.
After the test
- Record the patient ID, result, test name, lot, expiry date, date, time and your name.
- Dispose of the used device as infectious waste.
- If your national testing guidance requires a confirmatory step for certain results, follow it. Do not give a first result as final when confirmation is required.
Sources: WHO guidance on the use and quality assurance of rapid diagnostic tests (current edition) · Manufacturer's instructions for use for each RDT · National testing algorithms and guidelines of the Ministry of Health (current)
Quick check
For licensed professionals and students. Educational information only: always follow current national guidelines (EFDA, Ministry of Health), the product label and your facility’s procedures.
